From midnight today, traffic is set to return to normal.
The resumption of normal life in the city of 11 million people is seen as an important signal for China that the worst of the crisis is over.
The novel strain of coronavirus jumped from an unknown animal species to the human population in Wuhan in December.
Of the more than 80,000 officially registered cases in China, 50,000 were in Wuhan alone.
Observers believe that many cases have not been included in those numbers, however.